In the case of the 6 year old common bred cat presenting dispnoea for 3 weeks, while using the classical MGG coloration, at the microscopic examination of the smear that was made on a draw taken from an intensely hemorrhagic pleural effusion, there was discovered a neoplastic young cell population, with an intense anisocytosis, severe anisopoikilokaryosis, monstrous nuclei, multiple nuclei baring cells, an abundant and intense basophile cytoplasm and numerous atypical mitosis.
In conclusion, the cytopunction is characteristic for an intensely hemorrhagic effusion.
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The images are mainly compatible with a carcinoma or a mesothelioma. Following the immunocytomarking, the definite diagnosis was made, and consisted of a neoplastic pleural effusion associated to a pleural carcinoma Fig. A tumoral pleural effusion in the case of a cat.
Multiple nuclei-baring tumoral cells. Atypical mitosis. MGG, x Fig. A pleural effusion in a cat. Pleural carcinoma. ICC, x Positive reaction 2.
In the case of the recurring pleural effusion of a neoplastic type, from a 10 year old cat, using the MGG coloration, there was observed a moderate blood contamination and an inflammatory population composed of neutrophile granulocytes and macrophages. The macrophages present numerous cell detritus inside the cytoplasm. There are also present spherical cells of various sizes, with an intracytoplasmatic material mucus and numerous atypical mitosis — a cytological aspect in the favor of a malignant neoplastic effusion.
The 22 Lucrări Ştiinţifice — vol. A neoplastic pleural effusion in a cat. An eosinophilic matter abundently proteic material.
Active macrophages. Intracytoplasmatic cell detritus.
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MGG, x Dog food for prostate health immunocytomarking was proven to be inconclusive for the final diagnosis a suspicion of pleural carcinoma or mesothelioma because the material of the smear presented a dense film of an eosinophilic material proteins. A male 2 year old common bred cat presented a cranial mediastinal neoplasm, a cough and an occasional dispnoea.
Using the usual MGG coloration, the microscopic examination following the cytopunction of the mediastinal formation is characterized by a slight blood contamination without any erytrophagocytosis or hemosiderosis images. The main cells present an anaplastic character and are either isolated or disposed in intensely basophilic placard. They have a polyedric shape, a 25 to 40 µm diameter and show a strong anisocytosis with unclear cytoplasmatic limits.
The cytoplasm is abundent, the nucleus is oval, the chromatine is fine baring 1 to 2 large nucleoli and with a high power of anisopoikilokariosis. There are also seen remnants of free nuclei a nuclear fragility and small lymphocytes in a small number. In conclusion, the draw shows a malignant neoplastic formation, with an intensely anaplastic aspect dog food for prostate health is compatible with a malignant epithelial thymoma Fig. In the case of the draw that came from the analize psa si free psa effusion colored using MGG there can be observed a background of the smear that consists of a light granullar proteic dog food for prostate health and a slight blood contamination, without any signs of erytrophagocytosis or hemosiderosis.
The draw is composed especially of dog food for prostate health lymphocytes and undegenerated neutrophilic granullocytes, as well as several foam-shaped active macrophages. The cytological aspect is compatible to a modified transsudate or a starting chilothorax Fig. A mediastinal neoplasm in a cat.
Tumoral cells. Outstanding anisocytosis. Free nuclei.
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MGG, x 24 Lucrări Ştiinţifice — vol. A pleural neoplastic effusion in a cat. Foam-shaped active macrophages. Mature lymphocytes. Neutrophilic granullocytes. A negative control. The probe was poor in cells. Negative marking. Decreased cellularity. Negative marking - The cytological and immunocytochemical examination of the 3 draws from cavitary effusions evaluated in the course of this study have permitted the elaboration of the following main conclusions: 1.
In the case of the effusions that were associated to neoplastic paul pala tratează prostatita there were emphasised the malignancy criterias of tumoral cells: anisocytosis, anisocariosis, multiple nuclei-baring cells, large nucleoli and an intense vacuolisation of the cytoplasm.
In the case of the studied draws that were cytologically examined, there was not possible to identify the origin of tumoral cells because of the morphologic changes that they had suffered.
As follows, the draws were examined using the complementary immunocytochemical examination.
Using the immunocytochemical method, the malignant tumoral cells have been identified in a single case from the three that were evaluated the first case with the pleural carcinoma. In two of the cases, the immunocytomarking was negative because the specific antibodies were blocked by the intensely proteic background and the small number of cells from the draw, therefore the diagnosis consisted of suspicioned malignancy.
Combining the routine cytopathologic evaluation with an immunocytochemical examination can lead to a specific correct diagnosis, that can be used for the final treatment surgical treatment, a neohelping treatment or an aiming therapy and 26 Lucrări Ştiinţifice — vol. Bibliography 1. Cotea C. Embriologie generală.
Histologic generală, Editura Tehnopress, Iaşi, Garaud J. Lebascle Erwan G. Marluce Bibbo, M. Mary Anna Thrall - Cytology for Pathologists. Hoinghaus, M. Hewicker-Trautwein, R. Manolescu N. Curtea Veche, Bucureşti, Kefir is obtained after fermentation of milk at ambient temperatures for overnight and fermentation of the lactose yields a sour, carbonated, slightly alcoholic beverage 1.
Kefir contains beneficial bacteria help the digestive system run smoothly by improving digestion and further facilitates the absorption of the nutrients. Hens can not digest nutrients effectively especially proteins, so we aimed in this study to investigate the different levels of kefir in drinking water on the protein metabolism of the laying hens.
Following the incubation period for h, both gram - and lactophenol cotton blue staining methods were employed. For microscopic evaluations, microorganism identifications of the samples were then made using the conventional methods. Animals were fed for 11 weeks with basal diets complying with the NRC recommendations. Samples were taken for three times: At start, 5 th week and end of the experiment.
SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of serum, egg-yolk and egg-white proteins were conducted according to Laemmli 5. Lucrări Ştiinţifice — vol. There were 11, 23 and 24 proteins were detected in egg-white, serum and egg-yolk respectively.
Benha Uni. The vomeronasal organ of the Egyptian buffaloes was a paired tubular structure and had a considerable length, between 15 and 17 cm. The rostral part of the organ was lined by psudostratified epithelium ciliated with glandular area under it. An occasional goblet cell can dog food for prostate health seen in this area; the cytoplasm and ciliated border of these cells were strongly PAS-positive.
There was loose arrangement of glandular tubules in a rather closely woven fibrous to very dense fibrous tissue, which formed from collagen and elastic fiber in which were embedded numerous glands which serous or mucous either mixed type which gave positive reaction to the PAS, these glands may extend to the cartilage.
The glands open in the surface by pores. Several arteries and veins had relatively muscular walls. There was a network of small blood vessels very close to the basal lamina, sometimes so close that the vessels indent the lamina and push well up to the level of the basal cells.
Lamina propria submucosa was prostatita riciniol by blood vessels especially veins which were large and thinner ones also blood capillary which gave erectile appearance these vessels were irregularly distributed around vomeronasal duct.
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Introduction In the majority of mammals, odours are detected in two ways: by means of the main and the accessory olfactory systems. The previous of the two types commonly called the vomeronasal system share a common pattern dog food for prostate health organization, despite significant morphological and physiological differences, including the size of the structures of which they are formed Halpern, and Brennan, In microsmatic mammals including man, it reaches maximal development during foetal life and little remains in the adult.
In the domestic species, including horses, sheep, cattle, pigs and dogs, it remains an apparently functional organ of some size in the adult Kratzing, a. Around the vomeronasal duct there is a considerable amount of glands, vessels, nerves and connective tissue, which organize the soft tissue of the urinary retention medication organ VNO.
Additionally, a thick capsule wraps all these structures Vaccarezza, Sepich, and Tramezzani, and Keverne, The vomeronasal organ VNO is a chemosensory structure that has morphological indications of functionality and New World primates examined to date. In these species, it is thought to mediate certain socio-sexual behaviors. A recent study on the VNO in the chimpanzee Smith et al.
Many researchers described the vomeronasal organ in different species, bovine Adams, ; in pigs, cows and dog food for prostate health. Salazar, et. Al ; in porcine Ishida et al ; in rabbits Gaafar and in rat Garrosa, Picioare prostatita rece specimens were obtaining from Benha and Toukh slaughter houses, Egypt.
Immediately after slaughter the vomeronasal organ was located by sectioning the head transversely at the level of the first cheek tooth. The scanning electron microscopy was done at the Faculty of Agriculture, Alazhar University. The length of the vomeronasal organ was measured using SemAfore software. Result The vomeronasal organ of the Egyptian buffaloes is a paired tubular structure attached to the vomer bone and the floor of the nasal cavity, and is entirely covered by the corresponding mucosa Fig.
The vomeronasal organ has a considerable length, between 15 and 17 cm, and formed from several layers Fig. The rostral part of the organ is lined by psudostratified epithelium ciliated with glandular area under it. The epithelium over this glandular area is consists que es prostatitis crónica three types of cells forming two layers: a row of basal cells, then a more superficial zone consisting of ciliated cells Fig.
An occasional goblet cell can be seen in this area; the cytoplasm and dog food for prostate health border of these cells are strongly PAS-positive Fig. The epithelium is usually three to four nuclei in depth over the glandular area Fig. There was loose arrangement of glandular tubules in a rather dog food for prostate health woven fibrous Fig.
The glands open in the surface by pores Fig. Several arteries and veins have relatively muscular walls Fig. The epithelium of caudal portion of the organ is a row of basal cells with dark, rather irregularly shaped nuclei, and cytoplasm which is strongly eosinophilic. The next zone consists of larger, rounded cells with large pale nuclei. These cells show great variation in staining in different parts of the section; some are heavily stained, while in other areas both nucleus and cytoplasm are only lightly stained Fig Above these, there is a superficial zone of tall, columnar cells with long, ovoid nuclei and apical cytoplasm ending in a well-marked terminal bar and ciliated border.
There are some area have no glands, but in several places the epithelium dips down into a crypt-like formation lined with mucus-producing cells which stain a bright magenta with PAS technique Fig. Universitatea de Științe Agricole și Medicină Veterinară Iași In medial wall of vomeronasal organ was lined by olfactory epithelium or the sensory epithelium was showing the sustenticular cells, the receptor cells, their process and the basal cells Fig.
The basal lamina of the epithelium is not very well defined, even with PAS technique. Below it, very fine bundles of nerve fibres belonging to the vomeronasal nerve Fig. Larger bundles of these unmyelinated fibers can be seen deeper in the propria, close to the underlying cartilage. There is a network of small blood vessels very close to the basal lamina, sometimes so close that the vessels indent the lamina and push well up to the level of the basal cells. Lamina propria sub mucosa was characterized by blood vessels especially veins which are large and thinner ones also blood capillary which gave erectile appearance Fig 18 these vessels are irregularly distributed around vomeronasal duct.
Small arteries are usually seen near to the cartilage. The vomeronasal organ is completely surrounded by a vomeronasal cartilage. This cartilage is hyaline type Fig. Scale bar µm.
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Fig 2 : Scanning electron micrograph showing the Hyaline cartilage Hc of vomeronasal organ in ox, artery A and vein V.
Fig 3 : Scanning electron micrograph showing the cilia of the surface epithelium lining of vomeronasal organ in ox arrows. Scale bar 10 µm. Fig 4 : Scanning electron micrograph showing the secretory vesicles on the upper surface of vomeronasal organ of ox. Showing the psudostratified epithelium with pigment cell in between, cilia Ccellular lamina propria Lp and ill distinct basal lamina.
Scale bar 20 µm. Scale bar.