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Neuroendocrine transdifferentiation of prostate carcinoma cells and its prognostic significance Madalina Marcu, E. Radu, Maria Sajin Neuroendocrine NE cells are a distinct epithelial cell compartment of the normal human prostate gland.

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Their phenotype and range of endocrine secretion products are similar, but not identical to those of NE-like cells from prostate carcinoma. Neuroendocrine differentiation NED is a feature commonly seen in prostate carcinoma and a number of studies pointed out that its extent is associated to hormone therapy refractory and aggressive disease.

However, less information is available on the significance of NED in organ-confined prostate cancer, although identification of early predictors extract prostate tissue aggressive disease would obviously allow for more adequate therapy.

We review here recent advances in understanding the differentiation pathways of normal and malignant neuroendocrine cells as well as current information regarding the prognostic and therapeutic implication of NED assessment. The value of kidney biopsy in diabetes mellitus M. Penescu, E. Mandache The purpose of this work is to emphasize the value of kidney biopsy in patients with diabetes mellitus and clinical renal impairment.

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Multiple genetic predisposing conditions are involved in the development or not of a diabetic nephropathy, therefore supporting the existence of several factors in the pathogenesis of this disease.

These predisposing conditions may also favor different other types of glomerulonephritis which can occur independently or in parallel with a diabetic nephropathy.

All extract prostate tissue renal diseases have different treatments, and therefore they must be correctly identified and managed accordingly. The processing of the kidney biopsy samples requires a very careful and highly qualified management to differentiate precisely the nature of each condition.

In addition to the mesangial classical lesions, recent biopsy studies provided evidence that podocytes are injured very early in the diabetic nephropathy. On the other hand, transgenic mice models provide a unique opportunity to investigate the natural course of the disease. The paper underlines the main laboratory techniques required for this activity, and the main structural arguments to perform a satisfactory differential diagnosis.

Chronic complications in hemodialysis: correlations with primary renal disease I. Checherita, Flavia Turcu, R. Dragomirescu, A. Ciocalteu Although hemodialysis technique has improved in the last decades and the accessibility to this life-sustaining treatment modality increased rapidly, we are still concerned about the morbidity and mortality rates of dialysis patients. While technical advances are increasing the efficacy and safety of renal replacement therapies, latest studies are focused on other outcomes: increasing survival rates and the quality of life by an adequate management of the complications in chronic renal patients.

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This article reviews the complications of chronic hemodialysed patient with special considerations for the role of the primary renal disease that caused renal failure. Izvernariu, M. Raica Renal carcinomas are a heterogeneous group of tumors, difficult to classify and identify precisely. Since their prognosis depends very extract prostate tissue upon their type, precise diagnosis might mean the difference between therapeutic success and patient death. Cytokeratins are particularly useful for the identification of the epithelial nature of the tumors, because their expression is maintained extract prostate tissue in poorly differentiated tumors.

Monoclonal xp prostatita pe viata such as CK7 and CK20 stain different components of the renal tubular system and are a useful duo for the identification of the origin of the different tumors that might arise in the kidney.

Clear cells carcinomas had the less percentage of positive cells, whereas papillary carcinomas were positive in seven out of eight cases. No difference in the staining pattern was noticed between type I and type II papillary carcinomas. CK20 was negative in all cases studied.

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Panduru, D. Cimponeriu, M. Cruce, Daniela Adriana Ion, E. Mota, Maria Mota, C. Chivu, A. Covic Diabetic nephropathy is a major complication of type 1 diabetes whose pathogenesis is insufficiently known, but oxidative stress and genetic susceptibility seem to be involved.

There have been enrolled unrelated patients, having type 1 diabetes, divided into group A patients with diabetic nephropathy - macroalbuminuria or ESRD End Stage Renal Disease and group B patients without diabetic nephropathy. The statistical analysis has been made using De Finetti's extract prostate tissue. This study shows the association of a mutant C-allele of rs polymorphism in SOD1-gene with the advanced stages of diabetic nephropathy in patients with type 1 diabetes in Romania, suggesting the involvement extract prostate tissue the defense against oxidative stress, as an important link in the pathogeny of diabetic nephropathy.

Pavai, Extract prostate tissue Pap, K. Orban-Kis, T. Szilagyi Inhibition in the central nervous system is largely mediated by extract prostate tissue neurons that release GABA gamma-amino-butyric acid. GABAA-receptors play a major role in virtually all brain physiological functions and serve as targets for numerous classes of drugs, used both in clinical practice and as research tools. These receptors are heteropentamers, alpha1 extract prostate tissue the most widely occurring subunit; therefore it is the best candidate to be studied in pathological conditions where the inhibitory system might be altered e.

TaqMan probe was used in order to avoid detection of non-specific amplification products and synaptophysin as internal control. This substance was chosen because it has a stable expression restricted to neurons, and contrary to GAPDH, the most commonly used reference gene for expression analysis, synaptophysin expression is not modified in animal models of epilepsy.

Expression of synaptophysin was higher than expression of GABAAR-alpha1 in all samples from the central nervous system. The latter was significantly different among the studied brain areas. It was the smallest in the hippocampus, intermediate in the neocortex and the highest in the cerebellum.

Interanimal differences were small for any brain region under study.

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Capitanescu, Cristiana Simionescu, A. Stepan, Cl. Margaritescu, Claudia Valentina Georgescu, Anca Rosu In this paper, we studied 15 cases of early rheumatoid arthritis presenting with inflammatory lesions in different degrees of evolution. We want to highlight B- and T-lymphocytes in synovial tissue collected from patients diagnosed with early rheumatoid arthritis, to establish the pattern of their distribution, possibly in relation to local neovascularisation to determine the role played by these types of cells.

The pathological samples were represented by synovial membrane biopsy fragments, which were examined by histopathological and immunohistochemical methods.

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We noticed a perivascular distribution of lymphocyte infiltrate, up to formation of lymphoid follicles with germinal centers. There is a close interdependence between B- and T-lymphocytes in these lesions, and their presence in the synovial membrane in relation to newly formed blood vessels facilitates their action and their chemical mediators.

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Studying the interdependence of different types of lymphocytes and their connection with blood vessels may generate new therapeutic targets Corresponding author: Bogdan Capitanescu, University Assistant, MD, PhD, e-mail: bogdanc26 yahoo.

Neurodegenerative changes in human aging brain. An autopsy study D. Arsene, Carmen Ardeleanu Neurodegenerative pathological changes are known as occurring in human brain, in some way paralleling aging. We characterized prospectively the occurrence of cortical senile plaques and neurofibrillary tangles in 55 adult human subjects, by post-mortem examination.

We tried to determine if aging is associated with greater senile plaque and neurofibrillary tangles burden and what is the cortical distribution of lesions, regardless the mental status of the patient. The series comprised a large spectrum of ages, from 30 to year-old.

Immunohistochemistry for amyloid-beta Abeta and tau protein was the technique we used. ApoE genotyping was performed in 33 cases by polymerase chain reaction.

In our series brain Abeta deposition as senile plaques occurred only after year-old. These accumulations were strongly associated with the occurrence of neurofibrillary tangles. However, several very old patients were lacking both beta-amyloid and tau-positive lesions.

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As a result, even though Abeta and tau protein show a certain predilection for brain deposition in elder people, their relationship with aging still needs further investigation, mostly in human subjects.

The recesses of the retro-tympanum A. Material and Methods: Thirty-seven cadaver temporal bones were examined through the anterior dissection approach. The opening was enlarged by drilling to allow a good microscopic or rigid endoscopic view to the retro-tympanum.

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Measurements of the recesses were made with gradually millimeter wire, dial micrometric indicator comparator clockvarious right angled picks and sometimes by means of cast-impression - used in stomatology for dental impression interior configuration. Results: Out of 37 specimens, we have found PTS in For FR, we have found four types. The most constant cell, located in the inferior external part of FR and in the same time, the biggest one was suprapyramidal Sappey's fossa, which we have found in Sinus entrance of each recess was very variable, but in the most cases, we have found that the long diameter of ST and LTS was usually directed vertically and of PTS usually horizontally.

The deeper recess was ST - 2. In many cases, the inner configuration of each sinus was irregular, presenting either small recesses 0.

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Conclusions: Presence of the all four recess is not a rule. Marked variation in the size and shape and especially in the internal configuration of all recesses is a rule.

All recesses of the retro-tympanum can be recognized more easily, if we identify at first all elements of positive projection pyramidal eminence, chordal ridge, styloid eminence, styloid ridge, pyramidal ridge, suprapyramidal ridge, ponticulus, subiculum.

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Mucin is not a rare finding in rosacea A. Fernandez-Flores Mucin deposit is a feature that is not commonly mentioned in textbooks when talking about rosacea. Nevertheless, it is one of the prominent findings of a severe variant of the phymatous type of rosacea known as the fibrous type.

We retrospectively investigated 20 cases of early stages of rosacea and examined the mucin deposit in them, with histochemical stains Alcian Blue and Periodic Acid-Schiff.

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Alcian Blue positive deposits of mucin were found in all cases with granulomas. The mucin was located in the granulomas four cases as well as in the infundibulum one case. No deposits of mucin were evidenced in the dermis out of the granulomas, apart from the normal mucin of papillary and adventicial dermis.

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Periodic Acid-Schiff did not show any deposits in any case. Serologic lupus markers were negative in all patients with mucin deposits. We conclude that: a mucin is a common finding in granulomas of rosacea; b this mucin is probably not related to any progression to the mucinous variant of rhinophyma; c since discoid erythematosus lupus is a clinical differential of rosacea, it is important to be aware of the fact that mucin is a common finding in extract prostate tissue granulomas, in order not to misdiagnose both entities.

Eloae Zugun, E. Tarcoveanu, E. Twenty cases of colorectal liver metastases have been studied, including eight with recurrent metastases occurred after a previous treatment by thermonecrosis group 1 and 12 with primary metastases treated exclusively by surgery group 2.

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The cases were investigated by routine histopathological exam and by immunohistochemistry, using CD3, CD20 and CD68 antibodies. The design of the study envisages a comparative qualitative and quantitative evaluation of the B- and T-lymphocytes and macrophages inside the tumor and at the interface between liver parenchyma and tumor.

There are rumors that the tank seminal fluid can not maintain beyond a maximum threshold. Există zvonuri cum că rezervorul seminal nu poate menţine fluidul dincolo de un prag maxim. When sampling broodstock ovarian or seminal fluid may also be examined.

Student's t-test was used for all statistical comparisons. The qualitative exam revealed, for both groups, the presence of an important T-lymphocyte, and respectively B-lymphocyte cell population at the interface between the tumor and the liver parenchyma, the number of intratumoral cells being extremely reduced.

Grigoras Tie2 is a member of receptor tyrosine kinases family, involved in vasculogenesis and angiogenesis.

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Its main role is to stabilize, maintain, and facilitate the structural adaptation of the vasculature, during embryo development, and adult wound healing, or tumor development.